A simple high-performance liquid chromatographic method for estimating human serum angiotensin-converting enzyme activity

A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detec...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1985, Vol.3 (1), p.51-57
Hauptverfasser:	Maurich, V., Moneghini, M., Pitotti, A., Vio, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry angiotensin-converting enzyme assay Biological and medical sciences Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology hippuric acid determination Hydrolases Ion-pair high-performance liquid chromatography
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Zusammenfassung:	A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detection.
ISSN:	0731-7085 1873-264X
DOI:	10.1016/0731-7085(85)80006-6