Three-Dimensional Mammalian Cell Growth on Nonwoven Polyester Fabric Disks

Small disks of nonwoven polyester fabric (NWPF), similar to Fibra‐Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC‐5 cells. The disks can be autoclaved, and require inocula concentrations of only 2–5% of the final cell densities, which have reached 108 cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein reveal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.