Characterization of homogeneously staining regions in a small cell lung cancer cell line, using in situ hybridization with an MYCN probe

The cell line CIPL38 was derived from the pleural effusion of a patient with small cell lung cancer. The karyotype was hyperdiploid and complex with a variable number of marker chromosomes. Two of the markers had large homogeneously staining regions (hsr), which were shown to consist of amplified MYCN by in situ hybridization. One hsr bearing a marker chromosome could not be identified with G‐banding, but the other was situated on a der(14). This was elucidated further with FISH analysis, which enabled the identification of sequences of chromosome I involved in a complex rearrangement with chromosome 14 and the hsr. Genes Chromosom Cancer 10:213–216 (1994). © 1994 Wiley‐Liss, Inc.