Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii
A hypoxanthine-guanine phosphoribosyltransferase-encoding gene ( HGPRT) from Toxoplasma gondii has been isolated and sequenced. The identity of the enzyme was determined by sequence comparison and complementation in a mutant Escherichia coli.
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Veröffentlicht in: | Gene 1994-09, Vol.147 (1), p.153-154 |
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container_title | Gene |
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creator | Vasanthakumar, Geetha van Ginkel, Sabrina Parish, Gregory |
description | A hypoxanthine-guanine phosphoribosyltransferase-encoding gene (
HGPRT) from
Toxoplasma gondii has been isolated and sequenced. The identity of the enzyme was determined by sequence comparison and complementation in a mutant
Escherichia coli. |
doi_str_mv | 10.1016/0378-1119(94)90058-2 |
format | Article |
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HGPRT) from
Toxoplasma gondii has been isolated and sequenced. The identity of the enzyme was determined by sequence comparison and complementation in a mutant
Escherichia coli.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)90058-2</identifier><identifier>PMID: 8088544</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; complementation ; DNA, Complementary - genetics ; Genes, Protozoan ; HGPRT-deficient Escherichia coli ; Hypoxanthine Phosphoribosyltransferase - genetics ; molecular cloning ; Molecular Sequence Data ; pathogen ; purine pathway ; Recombinant DNA ; Toxoplasma - genetics ; Toxoplasma gondii ; λZAPII</subject><ispartof>Gene, 1994-09, Vol.147 (1), p.153-154</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-67aebdce52d1c747c774b8daae09df12c7d266ea82558ba97ea31fdd9e390fcc3</citedby><cites>FETCH-LOGICAL-c388t-67aebdce52d1c747c774b8daae09df12c7d266ea82558ba97ea31fdd9e390fcc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)90058-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8088544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vasanthakumar, Geetha</creatorcontrib><creatorcontrib>van Ginkel, Sabrina</creatorcontrib><creatorcontrib>Parish, Gregory</creatorcontrib><title>Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii</title><title>Gene</title><addtitle>Gene</addtitle><description>A hypoxanthine-guanine phosphoribosyltransferase-encoding gene (
HGPRT) from
Toxoplasma gondii has been isolated and sequenced. The identity of the enzyme was determined by sequence comparison and complementation in a mutant
Escherichia coli.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>complementation</subject><subject>DNA, Complementary - genetics</subject><subject>Genes, Protozoan</subject><subject>HGPRT-deficient Escherichia coli</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>pathogen</subject><subject>purine pathway</subject><subject>Recombinant DNA</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma gondii</subject><subject>λZAPII</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1r3DAQFaUl3ab9By3oVNqDG8myLelSCOlXICSX9CzG0nhXxZZcyS7Zfx9td8mxHRgGZt68Yd4j5C1nnzjj3QUTUlWcc_1BNx81Y62q6mdkw5XUFWNCPSebJ8hL8irnX6xE29Zn5Ewxpdqm2ZD1OscRFh8DheBoxt8rBuvDlsaBArVfbi9paUR3aC07pLv9HB8gLDsfsNquEEql8y7mksn3Me_HJUHIAybISIcUJ3ofH-I8Qp6AbmNw3r8mLwYYM7451XPy89vX-6sf1c3d9-ury5vKCqWWqpOAvbPY1o5b2UgrZdMrB4BMu4HXVrq66xBU3baqBy0RBB-c0yg0G6wV5-T9kXdOsTyWFzP5bHEcIWBcs5Gd5KJtuv8CedcpJoQuwOYItCnmnHAwc_ITpL3hzBxsMQfNzUFzoxvz1xZTl7V3J_61n9A9LZ18KPPPxzkWNf54TCZbX3RH5xPaxbjo_33gEVujn70</recordid><startdate>19940915</startdate><enddate>19940915</enddate><creator>Vasanthakumar, Geetha</creator><creator>van Ginkel, Sabrina</creator><creator>Parish, Gregory</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19940915</creationdate><title>Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii</title><author>Vasanthakumar, Geetha ; van Ginkel, Sabrina ; Parish, Gregory</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-67aebdce52d1c747c774b8daae09df12c7d266ea82558ba97ea31fdd9e390fcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>complementation</topic><topic>DNA, Complementary - genetics</topic><topic>Genes, Protozoan</topic><topic>HGPRT-deficient Escherichia coli</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>pathogen</topic><topic>purine pathway</topic><topic>Recombinant DNA</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma gondii</topic><topic>λZAPII</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vasanthakumar, Geetha</creatorcontrib><creatorcontrib>van Ginkel, Sabrina</creatorcontrib><creatorcontrib>Parish, Gregory</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasanthakumar, Geetha</au><au>van Ginkel, Sabrina</au><au>Parish, Gregory</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994-09-15</date><risdate>1994</risdate><volume>147</volume><issue>1</issue><spage>153</spage><epage>154</epage><pages>153-154</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A hypoxanthine-guanine phosphoribosyltransferase-encoding gene (
HGPRT) from
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ispartof | Gene, 1994-09, Vol.147 (1), p.153-154 |
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source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Amino Acid Sequence Animals Base Sequence Cloning, Molecular complementation DNA, Complementary - genetics Genes, Protozoan HGPRT-deficient Escherichia coli Hypoxanthine Phosphoribosyltransferase - genetics molecular cloning Molecular Sequence Data pathogen purine pathway Recombinant DNA Toxoplasma - genetics Toxoplasma gondii λZAPII |
title | Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii |
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