Biosynthetic pathway for a new series of gangliosides, GT1aα and GQ1bα

Biosynthetic pathway for a new series of gangliosides, GT1aα and GQ1bα

A new class of gangliosides, GT1aα and GQ1bα, were initially identified as cholinergic neuron-specific antigens in bovine brain. These gangliosides have in common α2–6 NeuAc linked to the GalNAc residue in the gangliotetraose core structure. In this study, we have determined the biosynthetic pathway...

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Veröffentlicht in:FEBS letters 1994-09, Vol.351 (2), p.291-294
Hauptverfasser: Irie, Fumitoshi, Jwa Hidari, Kazuya I.-P., Tai, Tadashi, Li, Yu-Teh, Seyama, Yousuke, Hirabayashi, Yoshio
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carbohydrate Sequence
Cattle
Cholinergic Fibers - chemistry
Cholinergic neuron-specific antigen
Female
Gal, galactose
GalNAc, N-acetylgalactosamine
Ganglioside
Ganglioside biosynthesis
Gangliosides - analysis
Gangliosides - biosynthesis
Glc, glucose
Golgi Apparatus - metabolism
HPTLC, high performance thin layer chromatography
Liver - enzymology
Molecular Sequence Data
NeuAc, N-acetylneuraminic acid
Rats
Sialyltransferase
Sialyltransferases - metabolism
Subcellular Fractions - metabolism
The nomenclature used for gangliosides is based on the system of Svennerhorm and Hirabayashi et al. [9,24]. Cer, ceramide
TLC, thin layer chromatography
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Zusammenfassung:A new class of gangliosides, GT1aα and GQ1bα, were initially identified as cholinergic neuron-specific antigens in bovine brain. These gangliosides have in common α2–6 NeuAc linked to the GalNAc residue in the gangliotetraose core structure. In this study, we have determined the biosynthetic pathways of GT1aα and GQ1bα using rat liver Golgi fraction. The results showed that GT1aα and GQ1bα were synthesized from GD1a and GT1b, respectively, by the action of a GalNAc α2–6 sialyltransferase. It was also demonstrated that these two gangliosides were found to exist as extremely minor components in rat liver.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00883-3