Endothelial desquamating activity of rat synthetic fibrinopeptide b and its analogues “in vivo” : Identification of responsible sequence

The endothelial desquamating activity of the synthetic rat fibrinopeptide B (ATTDSDKVDLSIAR-OH), and its analogues was studied “in vivo” after intravenous administration to rats. Rat fibrinopeptide B (FPB) caused a significant increase in the count of circulating endothelial cell carcasses at the dose of 100 nmol/kg. Maximal effect reaching about 270% of the normal value was achieved with the dose of 600 nmol/kg in 30 min. after the injection. No significant thrombocytopenia, no hemolysis and no other life-threatening complications were observed. The same endothelial desquamating effect was achieved with N-terminal FPB(1–7) peptide ATTDSDK-OH, but very low activity exhibited the two FPB mutant peptides: ATDSDKVDLSIAR-OH and ATTNSNK-OH. Our results indicate that N-terminal sequence (1–7) consisting of N-terminal “pig tail” (ATT), acid region (DSD) and basic aminoacid (K) is responsible for endothelial desquamating activity of rat FPB. Similar corresponding sequences may be reconized in FPB of different species. The conservation of this common “active site” sequence is less apparent in primates.