Homologous pairing of DNA molecules by ustilago rec1 protein is promoted by sequences of Z-DNA

Plasmids containing Z-DNA stretches can be paired and linked by combined action of Ustilago rec1 protein and topoisomerase. The product formed is a hemicatenated dimer in which two DNA rings are topologically intertwined at a region of homology. Superhelicity governs the reaction. Formation of linke...

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Veröffentlicht in:Cell 1986-02, Vol.44 (4), p.545-554
Hauptverfasser: Kmiec, Eric B., Holloman, William K.
Format: Artikel
Sprache:eng
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Zusammenfassung:Plasmids containing Z-DNA stretches can be paired and linked by combined action of Ustilago rec1 protein and topoisomerase. The product formed is a hemicatenated dimer in which two DNA rings are topologically intertwined at a region of homology. Superhelicity governs the reaction. Formation of linked product is coupled with formation of Z-DNA in the plasmid, a process dependent on the superhelix density. Pairing appears to initiate within the Z-DNA sequence, not at the unwound B-Z junction. The reaction can be blocked by a Z-DNA-specific binding protein, namely Z-DNA antibody. Plasmids with alternating Z-DNA dG-dC sequences at different sites on otherwise homologous molecules can be linked at the dG-dC sequences. However, a plasmid with a (dG-dC) n·(dG-dC) n Z-DNA stretch cannot be linked with a plasmid containing a (dG-dT) n·(dC-dA) n Z-DNA stretch.
ISSN:0092-8674
1097-4172
DOI:10.1016/0092-8674(86)90264-3