Studies of influenza haemagglutinin-mediated membrane fusion

A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer ( Biochemistry 24,1–8 (1985) ) was used in a study of influenza haemagglutininmediated fusion. The characteristics of fusion and haemolysis by X-31 (H 3N 2) virus, a number of mutants of X-31 which fuse me...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1986-02, Vol.149 (1), p.27-35
Hauptverfasser:	Wharton, S.A., Skehel, J.J., Wiley, D.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Viral Biological and medical sciences Fundamental and applied biological sciences. Psychology hemagglutinins Hemagglutinins, Viral - immunology Hemolysis Hydrogen-Ion Concentration influenza virus Kinetics Liposomes Membrane Fusion Microbiology Molecular Conformation mutants Mutation Orthomyxoviridae - genetics Orthomyxoviridae - immunology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Temperature Virology
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Zusammenfassung:	A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer ( Biochemistry 24,1–8 (1985) ) was used in a study of influenza haemagglutininmediated fusion. The characteristics of fusion and haemolysis by X-31 (H 3N 2) virus, a number of mutants of X-31 which fuse membranes at higher pH, and purified haemagglutinins released from virus particles either by detergent dissociation or by bromelain digestion were compared with particular regard to pH and temperature dependence. The finding that membrane fusion activity, haemolysis, and changes in haemagglutinin conformation covary with pH and temperature provide support for the role of haemagglutinin in fusion and are discussed in relation to the stability of its structure.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(86)90083-8