A simplified ELISA for anti-receptor antibodies in myasthenia gravis

Nicotinic acetylcholine receptor (nAchR) from triton extracts of muscle adsorbed specifically and optimally to microtitration plates at pH 7.4 rather than at pH 9.6. An ELISA for anti-receptor antibodies in myasthenia gravis based on direct adsorption of the receptor at pH 7.4 is described (direct a...

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Veröffentlicht in:	Journal of immunological methods 1986-01, Vol.86 (1), p.115-118
Hauptverfasser:	Jailkhani, Bansi L., Asthana, D., Jaffery, Nargis F., Kumar, Ramesh, Ahuja, G.K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption Acetylcholine receptor Autoantibodies - analysis Biological and medical sciences ELISA Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Hydrogen-Ion Concentration Molecular immunology Myasthenia gravis Myasthenia Gravis - immunology Receptors, Nicotinic - immunology Techniques
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Beschreibung
Zusammenfassung:	Nicotinic acetylcholine receptor (nAchR) from triton extracts of muscle adsorbed specifically and optimally to microtitration plates at pH 7.4 rather than at pH 9.6. An ELISA for anti-receptor antibodies in myasthenia gravis based on direct adsorption of the receptor at pH 7.4 is described (direct assay). The direct assay compares very well in sensitivity and specificity with an indirect assay, in which the receptor was attached through α-bungarotoxin adsorbed on the solid phase (correlation coefficient 0.94).
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(86)90273-5