Biochemical analysis of bovine testicular anti-Müllerian hormone

Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligo...

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Veröffentlicht in:FEBS letters 1986-01, Vol.195 (1), p.73-76
Hauptverfasser: Picard, Jean-Yves, Goulut, Chantal, Bourrillon, R., Josso, Nathalie
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Sprache:eng
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Zusammenfassung:Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligosaccharide composition suggests that both N- and O-glycosidically linked chains are present. The molecular extinction coefficient is 3.27 ± 0.06. One RIA unit, defined as the amount of hormone released by 1 g fetal bovine testicular tissue incubated during 4 h, corresponds to 3.06 ± 0.17 /smg protein.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)80133-8