Plasmid DNA Profiles as Epidemiological Markers for Clinical and Environmental Isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum

Plasmid DNA Profiles as Epidemiological Markers for Clinical and Environmental Isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum

Plasmid DNA was isolated, and profiles of a variety of clinical and environmental isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (MAIS) were compared to determine whether plasmid DNA content would be useful as an epidemiological marker for these environ...

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Veröffentlicht in:The Journal of infectious diseases 1986-02, Vol.153 (2), p.325-331
Hauptverfasser: Meissner, P. Scott, Falkinham, Joseph O.
Format: Artikel
Sprache:eng
Schlagworte:
Aerosols
Air Microbiology
Bacteriology
Biological and medical sciences
DNA, Bacterial - analysis
Environmental disorders
Environmental health
Environmental Microbiology
Epidemiology
Fundamental and applied biological sciences. Psychology
Genetics
Humans
Infections
Microbiology
Mycobacterium
Mycobacterium - genetics
Mycobacterium avium
Mycobacterium avium - genetics
Mycobacterium avium - isolation & purification
Mycobacterium Infections, Nontuberculous - microbiology
Mycobacterium intracellulare
Mycobacterium scrofulaceum
Nontuberculous Mycobacteria - genetics
Nontuberculous Mycobacteria - isolation & purification
Original Articles
Plasmids
Sedimentary soils
Sediments
Soil Microbiology
Soil water
Tuberculosis, Pulmonary - microbiology
Water Microbiology
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Zusammenfassung:Plasmid DNA was isolated, and profiles of a variety of clinical and environmental isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (MAIS) were compared to determine whether plasmid DNA content would be useful as an epidemiological marker for these environmental pathogens. Since plasmids are common in clinical isolates and are stable during culture and exposure to NaOH, plasmid DNA analysis appears to be a suitable epidemiological tool. Based on the high frequency of plasmids in only clinical (56%) and aerosol (75%) isolates and low frequencies in soil (5%), dust (7%), sediment (
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/153.2.325