A long range restriction map spanning the myxoid liposarcoma breakpoint in the q13-14 region of human chromosome 12

We have used pulsed-field gel electrophoresis to construct a long range restriction map of the myxoid liposarcoma (MLS) breakpoint region in 12q13-14. The CHOP/GADD153 gene, consistently translocated in myxoid liposarcomas, is located less than 55 kb from the putative oncogene GLI. We have used fluo...

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Veröffentlicht in:	Human genetics 1994-09, Vol.94 (3), p.259-264
Hauptverfasser:	Forus, A, Kools, P F, Schoenmakers, E F, Van de Ven, W J, Myklebost, O
Format:	Artikel
Sprache:	eng
Schlagworte:	CCAAT-Enhancer-Binding Proteins chromosome 12 Chromosome Aberrations Chromosome Banding Chromosomes, Human, Pair 12 DNA, Neoplasm - analysis Electrophoresis, Gel, Pulsed-Field Humans In Situ Hybridization, Fluorescence Liposarcoma, Myxoid - genetics man myxoid liposarcoma Oncogenes physical mapping Proteins - genetics restriction endonuclease mapping Restriction Mapping Transcription Factor CHOP Transcription Factors Tumor Cells, Cultured
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Zusammenfassung:	We have used pulsed-field gel electrophoresis to construct a long range restriction map of the myxoid liposarcoma (MLS) breakpoint region in 12q13-14. The CHOP/GADD153 gene, consistently translocated in myxoid liposarcomas, is located less than 55 kb from the putative oncogene GLI. We have used fluorescent in situ hybridization to orient the map with respect to the chromosome, and to show that GLI (and thus A2MR) is located proximal to the MLS breakpoint.
ISSN:	0340-6717 1432-1203
DOI:	10.1007/BF00208280