Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis

A proximal segment of B. subtilis sec Y gene was placed under the control of the inducible spac promoter Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via Campbell‐like reciprocal recombination. The growth of the resulting strain was strongly IPTG dependent. With staphylokinase and α‐amylase as reporter proteins it was found, that the protein secretion capacity of this strain was correlated to the conditions of repression or induction of the chromosomal spac promoter.