Effect of Phencyclidine on the Metabolism of Individual Brain Proteins

: The effect of phencyclidine on the metabolism of a selected number of rat brain proteins was determined using two‐dimensional gel electrophoresis and quantitative fluorography. When rats were injected with phencyclidine, modulation of individual protein metabolism occurred in the pituitary and cortex. That is, a few proteins showed increased and others decreased incorporation of [35S]methionine, whereas total protein metabolism was unaltered. In contrast, in vitro treatment of brain tissue with phencyclidine inhibited incorporation of ra‐diolabel into all proteins by — 50%, as shown by quantitative fluorography of individual proteins.