Phlebotomus perniciosus newstead, 1911, infection by various zymodemes of the Leishmania infantum complex in the Granada province (Southern Spain)

This study presents the results of the isoenzymatic characterization of 21 strains of Leishmania of sandfly ( P. perniciosus) origin from the Torvizcon area. It forms an integral part of a larger eco-epidemiological study of the Alpujarras (Granada province, Southern Spain). The strains analysed were shown to belong to the L. infantum complex based on the results of 15 enzymes. The electrophoretic profiles for the enzymes MDH, G6PD and NP1 have permitted the identification of four zymodemes: GR-1 (5 strains), GR-2 (2 strains), GR-3 (13 strains) and GR-7 (1 strain); only one of these zymodemes, GR-1, was found in the Torvizcon area in the vertebrate host (man and dog). This is the first time zymodeme GR-7 has been described.