Determination of sterol content in different food samples by capillary gas chromatography

An accurate method for the determination of sterols by capillary gas chromatography was developed and applied to the analysis of food. The procedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80°C and separation of the unsaponifiable matter...

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Veröffentlicht in:	Journal of Chromatography A 1994-06, Vol.672 (1), p.267-272
Hauptverfasser:	Rodríguez-Palmero, M., de la Presa-Owens, S., Castellote-Bargallo, A.I., Sabater, M.C.López, Rivero-Urgell, M., de la Torre-Boronat, M.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Chromatography, Gas - methods Fishes Food Analysis Food industries Fundamental and applied biological sciences. Psychology General aspects Humans Methods of analysis, processing and quality control, regulation, standards Poultry Sterols - analysis Vegetables
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Beschreibung
Zusammenfassung:	An accurate method for the determination of sterols by capillary gas chromatography was developed and applied to the analysis of food. The procedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80°C and separation of the unsaponifiable matter with cyclohexane, derivatization to form trimethylsilyl ethers and gas chromatography using 5α-cholestane as the internal standard. The method shows good accuracy, precision and sensitivity and is suitable for the determination of sterols in food.
ISSN:	0021-9673
DOI:	10.1016/0021-9673(94)80617-9