High-throughput rapid yeast DNA extraction: Application to yeast artificial chromosomes as polymerase chain reaction templates

An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is co...

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Veröffentlicht in:	Genetic analysis, techniques and applications techniques and applications, 1994, Vol.11 (1), p.7-11
Hauptverfasser:	Blanchard, Mary M., Nowotny, Volker
Format:	Artikel
Sprache:	eng
Schlagworte:	adn amplification chaine polymerase automation automatisation automatizacion chromosome chromosomes Chromosomes, Artificial, Yeast cromosomas dna DNA, Fungal - isolation & purification Polymerase Chain Reaction reaccion de cadenas de polimerasa saccharomyces cerevisiae
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Beschreibung
Zusammenfassung:	An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4°C is active in polymerase chain reaction experiments for more than a year.
ISSN:	1050-3862
DOI:	10.1016/1050-3862(94)90003-5