A study of the distribution of 7-ethoxycoumarin O-de-ethylase activity in human placental subcellular fractions
Human placental homogenates from maternal smokers and non-smokers were fractionated using differential centrifugation techniques. Yields of the various subfractions were determined and their homogeneity assessed using electron microscopic procedures. The distribution and response of 7-ethoxycoumarin...
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| Veröffentlicht in: | Placenta (Eastbourne) 1985-11, Vol.6 (6), p.481-495 |
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| creator | Kaelin, Alfred C. Fryer, Patricia R. Grindley, Helen Cummings, Alan J. |
| description | Human placental homogenates from maternal smokers and non-smokers were fractionated using differential centrifugation techniques. Yields of the various subfractions were determined and their homogeneity assessed using electron microscopic procedures. The distribution and response of 7-ethoxycoumarin
O-de-ethylase activity towards inhibition by dimethylsulphoxide, α-naphthoflavone and 9-hydroxyellipticine inhibitors in the placental subfractions were investigated. The low yield of microsomal protein obtained following differential centrifugation of placental homogenates (2.5±0.2 mg protein per g placenta) highlights the extremely refractory nature of human placental tissue towards homogenization.
Enzymic studies showed that the majority (75 per cent) of the original
O-de-ethylase activity in homogenates from smokers and non-smokers was to be found in the crude nuclear fraction. The 7-ethoxycoumarin
O-de-ethylase activity present in both homogenate and crude nuclear preparations from a maternal smoker was found to be inhibited by both α-naphthoflavone and 9-hydroxyellipticine to a lesser extent than the
O-de-ethylase activity which was present in both mitochondrial and microsomal fractions. While this observation suggests the existence of more than one induced
O-de-ethylase activity in the human placenta, the possibility that such differences in inhibitory response may be due to other factors (e.g. inhibitor solubility effects) cannot be excluded. |
| doi_str_mv | 10.1016/S0143-4004(85)80002-3 |
| format | Article |
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O-de-ethylase activity towards inhibition by dimethylsulphoxide, α-naphthoflavone and 9-hydroxyellipticine inhibitors in the placental subfractions were investigated. The low yield of microsomal protein obtained following differential centrifugation of placental homogenates (2.5±0.2 mg protein per g placenta) highlights the extremely refractory nature of human placental tissue towards homogenization.
Enzymic studies showed that the majority (75 per cent) of the original
O-de-ethylase activity in homogenates from smokers and non-smokers was to be found in the crude nuclear fraction. The 7-ethoxycoumarin
O-de-ethylase activity present in both homogenate and crude nuclear preparations from a maternal smoker was found to be inhibited by both α-naphthoflavone and 9-hydroxyellipticine to a lesser extent than the
O-de-ethylase activity which was present in both mitochondrial and microsomal fractions. While this observation suggests the existence of more than one induced
O-de-ethylase activity in the human placenta, the possibility that such differences in inhibitory response may be due to other factors (e.g. inhibitor solubility effects) cannot be excluded.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/S0143-4004(85)80002-3</identifier><identifier>PMID: 3879734</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>7-Alkoxycoumarin O-Dealkylase ; Benzoflavones - pharmacology ; Biological and medical sciences ; Cell Nucleus - enzymology ; Cytochrome P-450 Enzyme System - metabolism ; Dimethyl Sulfoxide - pharmacology ; Ellipticines - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Isoenzymes - metabolism ; Microscopy, Electron ; Microsomes - enzymology ; Mitochondria - enzymology ; Mother. Fetoplacental unit. Mammary gland. Milk ; Oxygenases - antagonists & inhibitors ; Oxygenases - metabolism ; Placenta - enzymology ; Placenta - ultrastructure ; Pregnancy ; Pregnancy. Parturition. Lactation ; Smoking ; Subcellular Fractions - enzymology ; Vertebrates: reproduction</subject><ispartof>Placenta (Eastbourne), 1985-11, Vol.6 (6), p.481-495</ispartof><rights>1986 W. B. Saunders Company Ltd.</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-6b7b7cd35f8bff3c44b1bfa6caf6fd3bcb9278964d8e086289052af83ad6ed8d3</citedby><cites>FETCH-LOGICAL-c389t-6b7b7cd35f8bff3c44b1bfa6caf6fd3bcb9278964d8e086289052af83ad6ed8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0143400485800023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8785210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3879734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaelin, Alfred C.</creatorcontrib><creatorcontrib>Fryer, Patricia R.</creatorcontrib><creatorcontrib>Grindley, Helen</creatorcontrib><creatorcontrib>Cummings, Alan J.</creatorcontrib><title>A study of the distribution of 7-ethoxycoumarin O-de-ethylase activity in human placental subcellular fractions</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Human placental homogenates from maternal smokers and non-smokers were fractionated using differential centrifugation techniques. Yields of the various subfractions were determined and their homogeneity assessed using electron microscopic procedures. The distribution and response of 7-ethoxycoumarin
O-de-ethylase activity towards inhibition by dimethylsulphoxide, α-naphthoflavone and 9-hydroxyellipticine inhibitors in the placental subfractions were investigated. The low yield of microsomal protein obtained following differential centrifugation of placental homogenates (2.5±0.2 mg protein per g placenta) highlights the extremely refractory nature of human placental tissue towards homogenization.
Enzymic studies showed that the majority (75 per cent) of the original
O-de-ethylase activity in homogenates from smokers and non-smokers was to be found in the crude nuclear fraction. The 7-ethoxycoumarin
O-de-ethylase activity present in both homogenate and crude nuclear preparations from a maternal smoker was found to be inhibited by both α-naphthoflavone and 9-hydroxyellipticine to a lesser extent than the
O-de-ethylase activity which was present in both mitochondrial and microsomal fractions. While this observation suggests the existence of more than one induced
O-de-ethylase activity in the human placenta, the possibility that such differences in inhibitory response may be due to other factors (e.g. inhibitor solubility effects) cannot be excluded.</description><subject>7-Alkoxycoumarin O-Dealkylase</subject><subject>Benzoflavones - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - enzymology</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Ellipticines - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>Microscopy, Electron</subject><subject>Microsomes - enzymology</subject><subject>Mitochondria - enzymology</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Oxygenases - antagonists & inhibitors</subject><subject>Oxygenases - metabolism</subject><subject>Placenta - enzymology</subject><subject>Placenta - ultrastructure</subject><subject>Pregnancy</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Smoking</subject><subject>Subcellular Fractions - enzymology</subject><subject>Vertebrates: reproduction</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHVKN2k_QmROFRVc3AKBht8qqIoaStFyqHtGfExaKm8Zgs4iv99cXa1155A8z7DDA9CV5TcUEL7Lz8J5azhhPDPsruWhJC2YW_QhnasXihp36LNCXmHLnL-U5mB0_YcnTMpBsH4BsVbnMvsFhw9LlvALuSSgplLiNNaEw2UbXxZbJx3OoUJPzUO1toy6gxY2xKeQ1lwTbaVmPB-1BamokecZ2NhHOdRJ-zTSsYpv0dnXo8ZPhzPS_T74f7X3ffm8enbj7vbx8YyOZSmN8II61jnpfGeWc4NNV73VvveO2asGVohh547CUT2rRxI12ovmXY9OOnYJfp0eHef4t8ZclG7kNd19ARxzkr0PRe8oxXsDqBNMecEXu1TqF9dFCVqFa1eRavVopKdehWtWO27Og6YzQ7cqetotuYfj7nOVo9VwGRDPmFSyK6lpGJfDxhUGc8Bkso2wGTBhQS2KBfDfxb5B64NnH0</recordid><startdate>19851101</startdate><enddate>19851101</enddate><creator>Kaelin, Alfred C.</creator><creator>Fryer, Patricia R.</creator><creator>Grindley, Helen</creator><creator>Cummings, Alan J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851101</creationdate><title>A study of the distribution of 7-ethoxycoumarin O-de-ethylase activity in human placental subcellular fractions</title><author>Kaelin, Alfred C. ; Fryer, Patricia R. ; Grindley, Helen ; Cummings, Alan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-6b7b7cd35f8bff3c44b1bfa6caf6fd3bcb9278964d8e086289052af83ad6ed8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>7-Alkoxycoumarin O-Dealkylase</topic><topic>Benzoflavones - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - enzymology</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>Ellipticines - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Isoenzymes - metabolism</topic><topic>Microscopy, Electron</topic><topic>Microsomes - enzymology</topic><topic>Mitochondria - enzymology</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Oxygenases - antagonists & inhibitors</topic><topic>Oxygenases - metabolism</topic><topic>Placenta - enzymology</topic><topic>Placenta - ultrastructure</topic><topic>Pregnancy</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Smoking</topic><topic>Subcellular Fractions - enzymology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaelin, Alfred C.</creatorcontrib><creatorcontrib>Fryer, Patricia R.</creatorcontrib><creatorcontrib>Grindley, Helen</creatorcontrib><creatorcontrib>Cummings, Alan J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaelin, Alfred C.</au><au>Fryer, Patricia R.</au><au>Grindley, Helen</au><au>Cummings, Alan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A study of the distribution of 7-ethoxycoumarin O-de-ethylase activity in human placental subcellular fractions</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>1985-11-01</date><risdate>1985</risdate><volume>6</volume><issue>6</issue><spage>481</spage><epage>495</epage><pages>481-495</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>Human placental homogenates from maternal smokers and non-smokers were fractionated using differential centrifugation techniques. Yields of the various subfractions were determined and their homogeneity assessed using electron microscopic procedures. The distribution and response of 7-ethoxycoumarin
O-de-ethylase activity towards inhibition by dimethylsulphoxide, α-naphthoflavone and 9-hydroxyellipticine inhibitors in the placental subfractions were investigated. The low yield of microsomal protein obtained following differential centrifugation of placental homogenates (2.5±0.2 mg protein per g placenta) highlights the extremely refractory nature of human placental tissue towards homogenization.
Enzymic studies showed that the majority (75 per cent) of the original
O-de-ethylase activity in homogenates from smokers and non-smokers was to be found in the crude nuclear fraction. The 7-ethoxycoumarin
O-de-ethylase activity present in both homogenate and crude nuclear preparations from a maternal smoker was found to be inhibited by both α-naphthoflavone and 9-hydroxyellipticine to a lesser extent than the
O-de-ethylase activity which was present in both mitochondrial and microsomal fractions. While this observation suggests the existence of more than one induced
O-de-ethylase activity in the human placenta, the possibility that such differences in inhibitory response may be due to other factors (e.g. inhibitor solubility effects) cannot be excluded.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>3879734</pmid><doi>10.1016/S0143-4004(85)80002-3</doi><tpages>15</tpages></addata></record> |
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| subjects | 7-Alkoxycoumarin O-Dealkylase Benzoflavones - pharmacology Biological and medical sciences Cell Nucleus - enzymology Cytochrome P-450 Enzyme System - metabolism Dimethyl Sulfoxide - pharmacology Ellipticines - pharmacology Female Fundamental and applied biological sciences. Psychology Humans Isoenzymes - metabolism Microscopy, Electron Microsomes - enzymology Mitochondria - enzymology Mother. Fetoplacental unit. Mammary gland. Milk Oxygenases - antagonists & inhibitors Oxygenases - metabolism Placenta - enzymology Placenta - ultrastructure Pregnancy Pregnancy. Parturition. Lactation Smoking Subcellular Fractions - enzymology Vertebrates: reproduction |
| title | A study of the distribution of 7-ethoxycoumarin O-de-ethylase activity in human placental subcellular fractions |
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