Inducible Inhibition of Eukaryotic Gene Expression

A regulatable binary expression system for eukaryotes was recently developed based on the tetracycline repressor and its operator. Here we show that this system can be successfully applied to express antisense RNA and completely inhibit gene expression in a tetracycline-repressible fashion.

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Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1994-08, Vol.202 (3), p.1664-1667
Hauptverfasser:	Pescini, R., Alouani, S., Proudfoot, A., Power, C., Mermod, J.J., Delamarter, J.F., Vanhuijsduijnen, R.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Complementary Gene Expression - genetics HeLa Cells Humans Receptors, Interleukin - genetics Receptors, Interleukin-8A Repressor Proteins - metabolism RNA, Antisense - genetics Tetracycline Transcription Factors - metabolism
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Beschreibung
Zusammenfassung:	A regulatable binary expression system for eukaryotes was recently developed based on the tetracycline repressor and its operator. Here we show that this system can be successfully applied to express antisense RNA and completely inhibit gene expression in a tetracycline-repressible fashion.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1994.2125