Cloning and expression of a novel human brain inward rectifier potassium channel
A complementary DNA encoding an inward rectifier K+ channel (HRK1) was isolated from human hippocampus using a 392-base pair cDNA (HHCMD37) as a probe. HRK1 shows sequence similarity to three recently cloned inwardly rectifying potassium channels (IRK1, GIRK1, and ROMK1, 60, 42, and 37%, respectivel...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-08, Vol.269 (32), p.20468-20474 |
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| container_title | The Journal of biological chemistry |
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| creator | MAKHINA, E. N KELLY, A. J LOPATIN, A. N MERCER, R. W NICHOLS, C. G |
| description | A complementary DNA encoding an inward rectifier K+ channel (HRK1) was isolated from human hippocampus using a 392-base pair
cDNA (HHCMD37) as a probe. HRK1 shows sequence similarity to three recently cloned inwardly rectifying potassium channels
(IRK1, GIRK1, and ROMK1, 60, 42, and 37%, respectively) and has a similar proposed topology of two membrane spanning domains
that correspond to the inner core structure of voltage gated K+ channels. When HRK1 was expressed in Xenopus oocytes, large
inward K+ currents were observed below the K+ reversal potential but very little outward K+ current was observed. In on-cell
membrane patches, single channel conductance (g) was estimated to be 10 picosiemens by both direct measurement and noise analysis,
in 102 mM external [K+]. HRK1 currents were blocked by external Ba2+ and Cs+ (K(0) = 183 microM, and K(-130) = 30 microM,
respectively), and internal tetraethylammonium ion (K(0) = 62 microM), but were insensitive to external tetraethylammonium
ion. The functional properties of HRK1 are very similar to those of glial cell inward rectifier K+ channels and HRK1 may represent
a glial cell inward rectifier. |
| doi_str_mv | 10.1016/S0021-9258(17)32016-1 |
| format | Article |
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cDNA (HHCMD37) as a probe. HRK1 shows sequence similarity to three recently cloned inwardly rectifying potassium channels
(IRK1, GIRK1, and ROMK1, 60, 42, and 37%, respectively) and has a similar proposed topology of two membrane spanning domains
that correspond to the inner core structure of voltage gated K+ channels. When HRK1 was expressed in Xenopus oocytes, large
inward K+ currents were observed below the K+ reversal potential but very little outward K+ current was observed. In on-cell
membrane patches, single channel conductance (g) was estimated to be 10 picosiemens by both direct measurement and noise analysis,
in 102 mM external [K+]. HRK1 currents were blocked by external Ba2+ and Cs+ (K(0) = 183 microM, and K(-130) = 30 microM,
respectively), and internal tetraethylammonium ion (K(0) = 62 microM), but were insensitive to external tetraethylammonium
ion. The functional properties of HRK1 are very similar to those of glial cell inward rectifier K+ channels and HRK1 may represent
a glial cell inward rectifier.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)32016-1</identifier><identifier>PMID: 8051145</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Animals ; Barium - pharmacology ; Base Sequence ; Biological and medical sciences ; brain ; Brain - metabolism ; cDNA ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Cesium - pharmacology ; Cloning, Molecular ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; hippocampus ; HRK1 gene ; Humans ; man ; Membrane Potentials - drug effects ; Molecular and cellular biology ; Molecular Sequence Data ; nucleotide sequence ; Potassium Channel Blockers ; potassium channels ; Potassium Channels - genetics ; Potassium Channels, Inwardly Rectifying ; prediction ; Sequence Homology, Amino Acid ; Xenopus</subject><ispartof>The Journal of biological chemistry, 1994-08, Vol.269 (32), p.20468-20474</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-62da06c412c8e478fbd64692a3e1e6d09be87013126fb89b6549d20df37f32683</citedby><cites>FETCH-LOGICAL-c440t-62da06c412c8e478fbd64692a3e1e6d09be87013126fb89b6549d20df37f32683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4259444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8051145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAKHINA, E. N</creatorcontrib><creatorcontrib>KELLY, A. J</creatorcontrib><creatorcontrib>LOPATIN, A. N</creatorcontrib><creatorcontrib>MERCER, R. W</creatorcontrib><creatorcontrib>NICHOLS, C. G</creatorcontrib><title>Cloning and expression of a novel human brain inward rectifier potassium channel</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>A complementary DNA encoding an inward rectifier K+ channel (HRK1) was isolated from human hippocampus using a 392-base pair
cDNA (HHCMD37) as a probe. HRK1 shows sequence similarity to three recently cloned inwardly rectifying potassium channels
(IRK1, GIRK1, and ROMK1, 60, 42, and 37%, respectively) and has a similar proposed topology of two membrane spanning domains
that correspond to the inner core structure of voltage gated K+ channels. When HRK1 was expressed in Xenopus oocytes, large
inward K+ currents were observed below the K+ reversal potential but very little outward K+ current was observed. In on-cell
membrane patches, single channel conductance (g) was estimated to be 10 picosiemens by both direct measurement and noise analysis,
in 102 mM external [K+]. HRK1 currents were blocked by external Ba2+ and Cs+ (K(0) = 183 microM, and K(-130) = 30 microM,
respectively), and internal tetraethylammonium ion (K(0) = 62 microM), but were insensitive to external tetraethylammonium
ion. The functional properties of HRK1 are very similar to those of glial cell inward rectifier K+ channels and HRK1 may represent
a glial cell inward rectifier.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Barium - pharmacology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>cDNA</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Cesium - pharmacology</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hippocampus</subject><subject>HRK1 gene</subject><subject>Humans</subject><subject>man</subject><subject>Membrane Potentials - drug effects</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Potassium Channel Blockers</subject><subject>potassium channels</subject><subject>Potassium Channels - genetics</subject><subject>Potassium Channels, Inwardly Rectifying</subject><subject>prediction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Xenopus</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFq3DAQhkVJSDebPkJAhxLag1uNLMvWMSxpUwi00AZ6E7I8ilVsaSOtm_btoyTL9lhdBjTfP8N8hJwD-wAM5MfvjHGoFG-6d9C-r3n5q-AVWQHr6qpu4OcRWR2Q1-Q051-sPKHghJx0rAEQzYp820wx-HBHTRgo_tkmzNnHQKOjhob4Gyc6LrMJtE_GB-rDg0kDTWh33nlMdBt3piSWmdrRhIDTGTl2Zsr4Zl_X5PbT1Y_NdXXz9fOXzeVNZYVgu0rywTBpBXDboWg71w9SSMVNjYByYKrHrmVQA5eu71QvG6EGzgZXt67msqvX5OJl7jbF-wXzTs8-W5wmEzAuWbdSljA0_wVBSq5kodekeQFtijkndHqb_GzSXw1MPynXz8r1k08NrX5WrqHkzvcLln7G4ZDaOy79t_u-ydZMLplgfT5ggjdKCPEPG_3d-OAT6t5HO-KsuVRlmeZMlLsfAfrvlA0</recordid><startdate>19940812</startdate><enddate>19940812</enddate><creator>MAKHINA, E. N</creator><creator>KELLY, A. J</creator><creator>LOPATIN, A. N</creator><creator>MERCER, R. W</creator><creator>NICHOLS, C. G</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940812</creationdate><title>Cloning and expression of a novel human brain inward rectifier potassium channel</title><author>MAKHINA, E. N ; KELLY, A. J ; LOPATIN, A. N ; MERCER, R. W ; NICHOLS, C. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-62da06c412c8e478fbd64692a3e1e6d09be87013126fb89b6549d20df37f32683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Barium - pharmacology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Brain - metabolism</topic><topic>cDNA</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Cesium - pharmacology</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hippocampus</topic><topic>HRK1 gene</topic><topic>Humans</topic><topic>man</topic><topic>Membrane Potentials - drug effects</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Potassium Channel Blockers</topic><topic>potassium channels</topic><topic>Potassium Channels - genetics</topic><topic>Potassium Channels, Inwardly Rectifying</topic><topic>prediction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAKHINA, E. N</creatorcontrib><creatorcontrib>KELLY, A. J</creatorcontrib><creatorcontrib>LOPATIN, A. N</creatorcontrib><creatorcontrib>MERCER, R. W</creatorcontrib><creatorcontrib>NICHOLS, C. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAKHINA, E. N</au><au>KELLY, A. J</au><au>LOPATIN, A. N</au><au>MERCER, R. W</au><au>NICHOLS, C. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression of a novel human brain inward rectifier potassium channel</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-08-12</date><risdate>1994</risdate><volume>269</volume><issue>32</issue><spage>20468</spage><epage>20474</epage><pages>20468-20474</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>A complementary DNA encoding an inward rectifier K+ channel (HRK1) was isolated from human hippocampus using a 392-base pair
cDNA (HHCMD37) as a probe. HRK1 shows sequence similarity to three recently cloned inwardly rectifying potassium channels
(IRK1, GIRK1, and ROMK1, 60, 42, and 37%, respectively) and has a similar proposed topology of two membrane spanning domains
that correspond to the inner core structure of voltage gated K+ channels. When HRK1 was expressed in Xenopus oocytes, large
inward K+ currents were observed below the K+ reversal potential but very little outward K+ current was observed. In on-cell
membrane patches, single channel conductance (g) was estimated to be 10 picosiemens by both direct measurement and noise analysis,
in 102 mM external [K+]. HRK1 currents were blocked by external Ba2+ and Cs+ (K(0) = 183 microM, and K(-130) = 30 microM,
respectively), and internal tetraethylammonium ion (K(0) = 62 microM), but were insensitive to external tetraethylammonium
ion. The functional properties of HRK1 are very similar to those of glial cell inward rectifier K+ channels and HRK1 may represent
a glial cell inward rectifier.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8051145</pmid><doi>10.1016/S0021-9258(17)32016-1</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Barium - pharmacology Base Sequence Biological and medical sciences brain Brain - metabolism cDNA Cell membranes. Ionic channels. Membrane pores Cell structures and functions Cesium - pharmacology Cloning, Molecular DNA, Complementary Fundamental and applied biological sciences. Psychology hippocampus HRK1 gene Humans man Membrane Potentials - drug effects Molecular and cellular biology Molecular Sequence Data nucleotide sequence Potassium Channel Blockers potassium channels Potassium Channels - genetics Potassium Channels, Inwardly Rectifying prediction Sequence Homology, Amino Acid Xenopus |
| title | Cloning and expression of a novel human brain inward rectifier potassium channel |
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