Non-muscle myosin heavy chain as a possible target for protein encoded by metastasis-related mts-1 gene

The mts-1 gene is associated with the expression of the metastatic phenotype of tumor cells. The protein product of the mts-1 gene belongs to the S100 family of Ca(2+)-binding proteins with unknown biochemical function. In the present work, monoclonal anti-Mts-1 antibodies were used to isolate and c...

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Veröffentlicht in:The Journal of biological chemistry 1994-08, Vol.269 (31), p.19679-19682
Hauptverfasser: KRIAJEVSKA, M. V, CARDENAS, M. N, GRIGORIAN, M. S, AMBARTSUMIAN, N. S, GEORGIEV, G. P, LUKANIDIN, E. M
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container_end_page 19682
container_issue 31
container_start_page 19679
container_title The Journal of biological chemistry
container_volume 269
creator KRIAJEVSKA, M. V
CARDENAS, M. N
GRIGORIAN, M. S
AMBARTSUMIAN, N. S
GEORGIEV, G. P
LUKANIDIN, E. M
description The mts-1 gene is associated with the expression of the metastatic phenotype of tumor cells. The protein product of the mts-1 gene belongs to the S100 family of Ca(2+)-binding proteins with unknown biochemical function. In the present work, monoclonal anti-Mts-1 antibodies were used to isolate and characterize Mts-1 protein possible targets. Mts-1 protein can be immunoprecipitated by both anti-Mts-1 and anti-myosin antibodies as a complex with myosin from lysates of different mouse and human cell lines. Precipitation of myosin by anti-Mts-1 antibodies is specific and depends on the presence of Mts-1 protein. Ca(2+)-dependent association between Mts-1 protein and the heavy chain of non-muscle myosin was demonstrated by blot overlay technique. Furthermore, association between myosin and Mts-1 was confirmed by sucrose gradient analysis. Finally, immunofluorescent staining of the mouse mammary adenocarcinoma cell line showed that Mts-1 protein is co-localized with the myosin complex. The data suggest that the target for Mts-1 protein is a heavy chain of non-muscle myosin.
doi_str_mv 10.1016/s0021-9258(17)32072-0
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Amino Acid Sequence
Animals
Binding Sites
Biological and medical sciences
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - metabolism
Cell physiology
Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Mice
Molecular and cellular biology
Molecular Sequence Data
Muscles - metabolism
Myosins - metabolism
Neoplasm Metastasis - genetics
S100 Calcium-Binding Protein A4
S100 Proteins
Tumor Cells, Cultured
title Non-muscle myosin heavy chain as a possible target for protein encoded by metastasis-related mts-1 gene
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