Antigenic Structure of the Coat Protein of Potato Mop-Top Furovirus

The antigenic structure of the coat protein (CP) of potato mop-top furovirus (PMTV) was studied by electron microscopy of virus particles labeled with gold-conjugated monoclonal antibodies (MAbs) and by the reactions of MAbs with overlapping octapeptides (Pepscan) representing the complete amino aci...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1994-09, Vol.203 (2), p.277-285
Hauptverfasser: Pereira, L.G., Torrance, L., Roberts, I.M., Harrison, B.D.
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Torrance, L.
Roberts, I.M.
Harrison, B.D.
description The antigenic structure of the coat protein (CP) of potato mop-top furovirus (PMTV) was studied by electron microscopy of virus particles labeled with gold-conjugated monoclonal antibodies (MAbs) and by the reactions of MAbs with overlapping octapeptides (Pepscan) representing the complete amino acid sequence of the CP. A total of seven epitopes were identified in the CP. MAb SCR 69 detected a continuous epitope, which was located at the extreme N-terminus of the CP, was exposed at the surface along the sides of PMTV particles, and was removed by treating them with trypsin. MAb SCR 68 detected a discontinuous epitope found at the concave end of PMTV particles. Five other epitopes, which were detected by Pepscan tests, were located internally in, and at intervals along, the CP amino acid sequence. A tentative model of the PMTV CP subunit was produced, based on computer-aided prediction of its secondary structure and apparent similarities with the CP of tobacco mosaic virus. In this model, four of the epitopes occur at high radius in each of the pairs of parallel and anti-parallel alpha-helices in the CP subunit. The fifth is at low radius in the putative left radial alpha-helix. The epitope detected by MAb SCR 77, although amenable to study by Pepscan, contains three reactive elements, separated by short runs of nonessential residues, in a sequence of 13 amino acids. In intact virus particles, the CPs of beet necrotic yellow vein furovirus and PMTV apparently differ in the accessibility of their N- and C-termini.
doi_str_mv 10.1006/viro.1994.1485
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A total of seven epitopes were identified in the CP. MAb SCR 69 detected a continuous epitope, which was located at the extreme N-terminus of the CP, was exposed at the surface along the sides of PMTV particles, and was removed by treating them with trypsin. MAb SCR 68 detected a discontinuous epitope found at the concave end of PMTV particles. Five other epitopes, which were detected by Pepscan tests, were located internally in, and at intervals along, the CP amino acid sequence. A tentative model of the PMTV CP subunit was produced, based on computer-aided prediction of its secondary structure and apparent similarities with the CP of tobacco mosaic virus. In this model, four of the epitopes occur at high radius in each of the pairs of parallel and anti-parallel alpha-helices in the CP subunit. The fifth is at low radius in the putative left radial alpha-helix. 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Psychology</subject><subject>FUROVIRUS DE LA MOPA DE LA PATATA</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGIE</subject><subject>INMUNOLOGIA</subject><subject>Microbiology</subject><subject>MODELE</subject><subject>MODELOS</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Plant Viruses - immunology</subject><subject>POTATO MOP TOP FUROVIRUS</subject><subject>potato mop-top virus</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>QUIMICA</subject><subject>Solanum tuberosum - microbiology</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LwzAYh4MoOj-uHgShB_HWmaRJmh5lOBUUB9vOIUnfamRrZpIO_O9t2fAmnsKb3_N-8CB0SfCYYCzuti74MakqNiZM8gM0IrgSOS4YOUQjjBnNhaT0BJ3G-In7uizxMTouOakkFiM0uW-Te4fW2WyeQmdTFyDzTZY-IJt4nbJZ8AlcO_zNfNLJZ69-ky_8Jpt2wffbu3iOjhq9inCxf8_QcvqwmDzlL2-Pz5P7l9wyIlOudVUYJpih0gAz1lJMhKHGGFvLgpdWsIZqIYvGABUWF4UmugbBOCeYk7o4Q7e7uZvgvzqISa1dtLBa6RZ8F1UpBGWUiX9BIrgkVMoeHO9AG3yMARq1CW6tw7ciWA161aBXDXrVoLdvuN5P7swa6l9877PPb_a5jlavmqBb6-IvxijlZTXsvdphjfZKv4ceWc4rTrjEuA_lLoTe5dZBUNE6aC3ULoBNqvbur_N-AI3mnaU</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Pereira, L.G.</creator><creator>Torrance, L.</creator><creator>Roberts, I.M.</creator><creator>Harrison, B.D.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940901</creationdate><title>Antigenic Structure of the Coat Protein of Potato Mop-Top Furovirus</title><author>Pereira, L.G. ; Torrance, L. ; Roberts, I.M. ; Harrison, B.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-aa93b464b28be4bcc2016b2bbbcd8357c64f2a683fbe26c033a1ade64551051d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Agronomy. 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Psychology</topic><topic>FUROVIRUS DE LA MOPA DE LA PATATA</topic><topic>Immunohistochemistry</topic><topic>IMMUNOLOGIE</topic><topic>INMUNOLOGIA</topic><topic>Microbiology</topic><topic>MODELE</topic><topic>MODELOS</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Plant Viruses - immunology</topic><topic>POTATO MOP TOP FUROVIRUS</topic><topic>potato mop-top virus</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>QUIMICA</topic><topic>Solanum tuberosum - microbiology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, L.G.</creatorcontrib><creatorcontrib>Torrance, L.</creatorcontrib><creatorcontrib>Roberts, I.M.</creatorcontrib><creatorcontrib>Harrison, B.D.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, L.G.</au><au>Torrance, L.</au><au>Roberts, I.M.</au><au>Harrison, B.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenic Structure of the Coat Protein of Potato Mop-Top Furovirus</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>203</volume><issue>2</issue><spage>277</spage><epage>285</epage><pages>277-285</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The antigenic structure of the coat protein (CP) of potato mop-top furovirus (PMTV) was studied by electron microscopy of virus particles labeled with gold-conjugated monoclonal antibodies (MAbs) and by the reactions of MAbs with overlapping octapeptides (Pepscan) representing the complete amino acid sequence of the CP. A total of seven epitopes were identified in the CP. MAb SCR 69 detected a continuous epitope, which was located at the extreme N-terminus of the CP, was exposed at the surface along the sides of PMTV particles, and was removed by treating them with trypsin. MAb SCR 68 detected a discontinuous epitope found at the concave end of PMTV particles. Five other epitopes, which were detected by Pepscan tests, were located internally in, and at intervals along, the CP amino acid sequence. A tentative model of the PMTV CP subunit was produced, based on computer-aided prediction of its secondary structure and apparent similarities with the CP of tobacco mosaic virus. In this model, four of the epitopes occur at high radius in each of the pairs of parallel and anti-parallel alpha-helices in the CP subunit. The fifth is at low radius in the putative left radial alpha-helix. The epitope detected by MAb SCR 77, although amenable to study by Pepscan, contains three reactive elements, separated by short runs of nonessential residues, in a sequence of 13 amino acids. In intact virus particles, the CPs of beet necrotic yellow vein furovirus and PMTV apparently differ in the accessibility of their N- and C-termini.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7519806</pmid><doi>10.1006/viro.1994.1485</doi><tpages>9</tpages></addata></record>
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subjects Agronomy. Soil science and plant productions
Amino Acid Sequence
Antibodies, Monoclonal - immunology
ANTIGENE
ANTIGENOS
Biological and medical sciences
Capsid - immunology
CHIMIE
Epitopes
Fundamental and applied biological sciences. Psychology
FUROVIRUS DE LA MOPA DE LA PATATA
Immunohistochemistry
IMMUNOLOGIE
INMUNOLOGIA
Microbiology
MODELE
MODELOS
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
Plant Viruses - immunology
POTATO MOP TOP FUROVIRUS
potato mop-top virus
PROTEINAS
PROTEINE
QUIMICA
Solanum tuberosum - microbiology
Virology
title Antigenic Structure of the Coat Protein of Potato Mop-Top Furovirus
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