Purification and characterization of chitinase from Candida albicans
A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacry...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 1994-06, Vol.119 (1‐2), p.111-117 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 117 |
|---|---|
| container_issue | 1‐2 |
| container_start_page | 111 |
| container_title | FEMS microbiology letters |
| container_volume | 119 |
| creator | Mellor, Keely J. Nicholas, Richard O. Adams, David J. |
| description | A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacrylamide gel run under denaturing conditions. Purified chitinase had pH and temperature optima of 5.0 and 50°C, respectively. Inhibition of enzyme activity by allosamidin was pH‐dependent occuring maximally at pH 8.0. Phospholipids had similar marked and highly specific effects on the activities of both the purified soluble enzyme and a solubilized microsomal chitinase from C. albicans. Evidence is provided for the existence of a complex chitinolytic system in this organism. |
| doi_str_mv | 10.1111/j.1574-6968.1994.tb06876.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76620286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76620286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4341-4f0d9f5af64869716b556a06f6745730ec7d7d65aaaec7390dd21b9ad79267ff3</originalsourceid><addsrcrecordid>eNqVkF1r2zAUhsXoSNN0P6FgStmdXcmWj6xdFErWbIWM7WK7Fsf6oAqO3UkOS_rrpxCT--pG4rzPK4mHkFtGC5bW_aZgteA5SGgKJiUvxpZCI6DYfyDzc3RB5rQSTc6oFJfkKsYNpZSXFGZk1tBKApdz8vXXLnjnNY5-6DPsTaZfMKAebfBvp-Hg0syPvsdoMxeGbbZMnDeYYdemZh-vyUeHXbSfpn1B_qyefi-_5-uf356Xj-tc84qznDtqpKvRAW9ACgZtXQNScCB4LSpqtTDCQI2I6VhJakzJWolGyBKEc9WCfD7d-xqGvzsbR7X1Uduuw94Ou6gEQEnLBhL45QTqMMQYrFOvwW8xHBSj6qhQbdTRkzp6UkeFalKo9ql8M72ya7fWnKuTs5TfTTlGjZ0L2GsfzxhnTDZ1lbCHE_bPd_bwjg-o1Y91Iqr_QOOOEg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76620286</pqid></control><display><type>article</type><title>Purification and characterization of chitinase from Candida albicans</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><source>Oxford University Press Journals Digital Archive Legacy</source><creator>Mellor, Keely J. ; Nicholas, Richard O. ; Adams, David J.</creator><creatorcontrib>Mellor, Keely J. ; Nicholas, Richard O. ; Adams, David J.</creatorcontrib><description>A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacrylamide gel run under denaturing conditions. Purified chitinase had pH and temperature optima of 5.0 and 50°C, respectively. Inhibition of enzyme activity by allosamidin was pH‐dependent occuring maximally at pH 8.0. Phospholipids had similar marked and highly specific effects on the activities of both the purified soluble enzyme and a solubilized microsomal chitinase from C. albicans. Evidence is provided for the existence of a complex chitinolytic system in this organism.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1994.tb06876.x</identifier><identifier>PMID: 8039649</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Candida albicans ; Candida albicans - enzymology ; Chitinases - isolation & purification ; Chromatography, Ion Exchange ; Complex chitinolytic system ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Hydrogen-Ion Concentration ; Microbiology ; Mycology ; Phospholipid ; Phospholipids - pharmacology ; Purified chitinase</subject><ispartof>FEMS microbiology letters, 1994-06, Vol.119 (1‐2), p.111-117</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4341-4f0d9f5af64869716b556a06f6745730ec7d7d65aaaec7390dd21b9ad79267ff3</citedby><cites>FETCH-LOGICAL-c4341-4f0d9f5af64869716b556a06f6745730ec7d7d65aaaec7390dd21b9ad79267ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1994.tb06876.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1994.tb06876.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4119853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8039649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mellor, Keely J.</creatorcontrib><creatorcontrib>Nicholas, Richard O.</creatorcontrib><creatorcontrib>Adams, David J.</creatorcontrib><title>Purification and characterization of chitinase from Candida albicans</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacrylamide gel run under denaturing conditions. Purified chitinase had pH and temperature optima of 5.0 and 50°C, respectively. Inhibition of enzyme activity by allosamidin was pH‐dependent occuring maximally at pH 8.0. Phospholipids had similar marked and highly specific effects on the activities of both the purified soluble enzyme and a solubilized microsomal chitinase from C. albicans. Evidence is provided for the existence of a complex chitinolytic system in this organism.</description><subject>Biological and medical sciences</subject><subject>Candida albicans</subject><subject>Candida albicans - enzymology</subject><subject>Chitinases - isolation & purification</subject><subject>Chromatography, Ion Exchange</subject><subject>Complex chitinolytic system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Phospholipid</subject><subject>Phospholipids - pharmacology</subject><subject>Purified chitinase</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1r2zAUhsXoSNN0P6FgStmdXcmWj6xdFErWbIWM7WK7Fsf6oAqO3UkOS_rrpxCT--pG4rzPK4mHkFtGC5bW_aZgteA5SGgKJiUvxpZCI6DYfyDzc3RB5rQSTc6oFJfkKsYNpZSXFGZk1tBKApdz8vXXLnjnNY5-6DPsTaZfMKAebfBvp-Hg0syPvsdoMxeGbbZMnDeYYdemZh-vyUeHXbSfpn1B_qyefi-_5-uf356Xj-tc84qznDtqpKvRAW9ACgZtXQNScCB4LSpqtTDCQI2I6VhJakzJWolGyBKEc9WCfD7d-xqGvzsbR7X1Uduuw94Ou6gEQEnLBhL45QTqMMQYrFOvwW8xHBSj6qhQbdTRkzp6UkeFalKo9ql8M72ya7fWnKuTs5TfTTlGjZ0L2GsfzxhnTDZ1lbCHE_bPd_bwjg-o1Y91Iqr_QOOOEg</recordid><startdate>199406</startdate><enddate>199406</enddate><creator>Mellor, Keely J.</creator><creator>Nicholas, Richard O.</creator><creator>Adams, David J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199406</creationdate><title>Purification and characterization of chitinase from Candida albicans</title><author>Mellor, Keely J. ; Nicholas, Richard O. ; Adams, David J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4341-4f0d9f5af64869716b556a06f6745730ec7d7d65aaaec7390dd21b9ad79267ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Candida albicans</topic><topic>Candida albicans - enzymology</topic><topic>Chitinases - isolation & purification</topic><topic>Chromatography, Ion Exchange</topic><topic>Complex chitinolytic system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Phospholipid</topic><topic>Phospholipids - pharmacology</topic><topic>Purified chitinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mellor, Keely J.</creatorcontrib><creatorcontrib>Nicholas, Richard O.</creatorcontrib><creatorcontrib>Adams, David J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mellor, Keely J.</au><au>Nicholas, Richard O.</au><au>Adams, David J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of chitinase from Candida albicans</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1994-06</date><risdate>1994</risdate><volume>119</volume><issue>1‐2</issue><spage>111</spage><epage>117</epage><pages>111-117</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacrylamide gel run under denaturing conditions. Purified chitinase had pH and temperature optima of 5.0 and 50°C, respectively. Inhibition of enzyme activity by allosamidin was pH‐dependent occuring maximally at pH 8.0. Phospholipids had similar marked and highly specific effects on the activities of both the purified soluble enzyme and a solubilized microsomal chitinase from C. albicans. Evidence is provided for the existence of a complex chitinolytic system in this organism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8039649</pmid><doi>10.1111/j.1574-6968.1994.tb06876.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1994-06, Vol.119 (1‐2), p.111-117 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76620286 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford University Press Journals Digital Archive Legacy |
| subjects | Biological and medical sciences Candida albicans Candida albicans - enzymology Chitinases - isolation & purification Chromatography, Ion Exchange Complex chitinolytic system Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hydrogen-Ion Concentration Microbiology Mycology Phospholipid Phospholipids - pharmacology Purified chitinase |
| title | Purification and characterization of chitinase from Candida albicans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T08%3A18%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20characterization%20of%20chitinase%20from%20Candida%20albicans&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Mellor,%20Keely%20J.&rft.date=1994-06&rft.volume=119&rft.issue=1%E2%80%902&rft.spage=111&rft.epage=117&rft.pages=111-117&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.1994.tb06876.x&rft_dat=%3Cproquest_cross%3E76620286%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76620286&rft_id=info:pmid/8039649&rfr_iscdi=true |