Purification and characterization of chitinase from Candida albicans

A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacry...

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Veröffentlicht in:	FEMS microbiology letters 1994-06, Vol.119 (1‐2), p.111-117
Hauptverfasser:	Mellor, Keely J., Nicholas, Richard O., Adams, David J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Candida albicans Candida albicans - enzymology Chitinases - isolation & purification Chromatography, Ion Exchange Complex chitinolytic system Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hydrogen-Ion Concentration Microbiology Mycology Phospholipid Phospholipids - pharmacology Purified chitinase
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Beschreibung
Zusammenfassung:	A novel procedure was used to purify a cytosolic chitinase from Candida albicans to electrophoretic homogeneity. The results represent the first demonstration of the purification of a fungal intracellular chitinase using the criterion of a single band detected following silver‐staining of a polyacrylamide gel run under denaturing conditions. Purified chitinase had pH and temperature optima of 5.0 and 50°C, respectively. Inhibition of enzyme activity by allosamidin was pH‐dependent occuring maximally at pH 8.0. Phospholipids had similar marked and highly specific effects on the activities of both the purified soluble enzyme and a solubilized microsomal chitinase from C. albicans. Evidence is provided for the existence of a complex chitinolytic system in this organism.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1994.tb06876.x