EFFECTS OF HYPERTHERMIA ON THE PROLIFERATION AND VIABILITY OF CULTURED HUMAN HEPATOBILIARY CARCINOMA CELLS

Proliferation and viability of the cultured human hepatobiliary carcinoma cells under hyperthermic culture conditions were examined using a newly established temperature gradient incubator. JHH-2 hepatoma cells and OZ bile duct carcinoma cells could not proliferate at 40°C and could not survive at 41°C. NOZAWA gallbladder carcinoma cells could proliferate at 41°C but could not survive at 42°C. JHH-2 and OZ were more susceptible to hyperthermia than normal diploid fibroblasts. On the observation under phase contrast microscope, the cells became larger and less dense at 41-42°C for 1-2 days. The cell shape were destroyed at 43°C for 1-2 days.