Characterization of two groEL genes in Streptomyces coelicolor A3 (2)

Two Streptomyces coelicolor A3(2) groEL-like genes, groEL1 and groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that groEL1, but not groEL2, was preceded...

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Veröffentlicht in:Gene 1994-06, Vol.144 (1), p.97-101
Hauptverfasser: Duchêne, Anne-Marie, Kieser, Helen M., Hopwood, David A., Thompson, Charles J., Mazodier, Philippe
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Sprache:eng
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Zusammenfassung:Two Streptomyces coelicolor A3(2) groEL-like genes, groEL1 and groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that groEL1, but not groEL2, was preceded by a groES-like gene. Northern blots showed that heat shock induced the accumulation of transcripts corresponding to groES (0.7 kb), groES/EL1 (2.3 kb) and groEL2 (2.1 kb). Unique transcription start points and promoters were located for groES/EL1 and groEL2, having −10 and −35 hexamers similar to eubacterial vegetative promoters. Regions located 5' to the groES/EL1 or groEL2 structural genes contain ‘GCACTCN 9GAGTGC’ motifs conserved upstream from the heat-shock genes of other bacteria.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)90210-0