Characterization of two groEL genes in Streptomyces coelicolor A3 (2)
Two Streptomyces coelicolor A3(2) groEL-like genes, groEL1 and groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that groEL1, but not groEL2, was preceded...
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Veröffentlicht in: | Gene 1994-06, Vol.144 (1), p.97-101 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Two
Streptomyces coelicolor A3(2)
groEL-like genes,
groEL1 and
groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that
groEL1, but not
groEL2, was preceded by a
groES-like gene. Northern blots showed that heat shock induced the accumulation of transcripts corresponding to
groES (0.7 kb),
groES/EL1 (2.3 kb) and
groEL2 (2.1 kb). Unique transcription start points and promoters were located for
groES/EL1 and
groEL2, having −10 and −35 hexamers similar to eubacterial vegetative promoters. Regions located 5' to the
groES/EL1 or
groEL2 structural genes contain ‘GCACTCN
9GAGTGC’ motifs conserved upstream from the heat-shock genes of other bacteria. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(94)90210-0 |