Vectors to facilitate the creation of translational fusions to the maltose-binding protein of Escherichia coli

A set of vectors has been constructed to facilitate the fusion of heterologous sequences to the C terminus of the maltose-binding protein (MBP) of Escherichia coli. The plasmids carry a cloning region comprising two blunt cloning sites, a BamHI site and multiple stop codons, and this has been placed...

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Veröffentlicht in:	Gene 1994-06, Vol.144 (1), p.69-73
Hauptverfasser:	Aitken, Robert, Gilchrist, Janice, Sinclair, M.Catherine
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence ATP-Binding Cassette Transporters Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Carrier Proteins - genetics Carrier Proteins - metabolism DNA, Recombinant Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins Expression Genetic Vectors Maltose-Binding Proteins MBP Molecular Sequence Data Monosaccharide Transport Proteins Protein Biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism staphylococcal enterotoxin A
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Beschreibung
Zusammenfassung:	A set of vectors has been constructed to facilitate the fusion of heterologous sequences to the C terminus of the maltose-binding protein (MBP) of Escherichia coli. The plasmids carry a cloning region comprising two blunt cloning sites, a BamHI site and multiple stop codons, and this has been placed in each reading frame so that translational fusions to MBP can be generated and manipulated with ease. To demonstrate the utility of this system, recombinant proteins have been engineered in which staphylococcal enterotoxin A has been fused to MBP.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(94)90205-4