The purification of urease from Aspergillus nidulans

1. 1. A purification procedure is described for Aspergillus urease, the most important step being affinity chromatography on hydroxyurea Sepharose. The enzyme exists as a single active species of M r240,000, 2. 2. The pure enzyme has an activity of 670 μmol urea hydrolysed/min, has a K m of 10 −3M, an optimum pH of 8.5 and a sub-unit M r, of 40,000.