Na/H exchange at the apical membrane of guinea-pig gallbladder epithelium: properties and inhibition by cyclic AMP

The properties and, in particular, the cAMP-sensitivity of apical Na/H exchange in guinea-pig gallbladder epithelium were investigated using gravimetric, pH-stat, and microelectrode techniques. Proton secretion was Na-dependent, inhibited by ouabain and amiloride, and insensitive to changes in apica...

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Veröffentlicht in:	Pflügers Archiv 1985-01, Vol.405 Suppl 1 (S1), p.S115-S120
Hauptverfasser:	Petersen, K U, Wehner, F, Winterhager, J M
Format:	Artikel
Sprache:	eng
Schlagworte:	8-Bromo Cyclic Adenosine Monophosphate - pharmacology Alprostadil - pharmacology Amiloride - pharmacology Animals Carrier Proteins - metabolism Cell Membrane - drug effects Cell Membrane - metabolism Cyclic AMP - physiology Epithelium - drug effects Epithelium - metabolism Gallbladder - metabolism Guinea Pigs Hydrogen-Ion Concentration Kinetics Membrane Potentials - drug effects Sodium - metabolism Sodium-Hydrogen Exchangers
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Zusammenfassung:	The properties and, in particular, the cAMP-sensitivity of apical Na/H exchange in guinea-pig gallbladder epithelium were investigated using gravimetric, pH-stat, and microelectrode techniques. Proton secretion was Na-dependent, inhibited by ouabain and amiloride, and insensitive to changes in apical membrane potential. It was markedly reduced by 8-Br-cAMP and PGE1. PGE1 also attenuated the changes in intracellular Na activity produced by luminal Na removal and restoration. Our results suggest inhibition of Na/H exchange by cAMP. In conjunction with the cAMP-induced rise in apical membrane Cl permeability shown previously, this effect can account for inhibition of NaCl absorption by cAMP.
ISSN:	0031-6768 1432-2013
DOI:	10.1007/BF00581791