A murine monoclonal antibody directed against a yeast cell wall glycoprotein antigen of the yeast genus Saccharomyces

A murine monoclonal antibody directed against a yeast cell wall glycoprotein antigen of the yeast genus Saccharomyces

Murine monoclonal antibodies (mAbs) were selected against a cell wall glycoprotein of Saccharomyces cerevisiae. One of the mAbs (92‐276/018) specifically identified S. cerevisiae and the sibling species S. paradoxus, S. pastorianus and S. bayanus in immunofluorescence studies and immunoblot analyses...

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Veröffentlicht in:FEMS microbiology letters 1994-05, Vol.118 (3), p.297-304
Hauptverfasser: Bröker, Michael, Harthus, Hans‐Peter, Barnes, Roger M.R.
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies, Fungal - immunology
Antibodies, Monoclonal
Antibody Specificity
Antigens, Fungal - immunology
Binding, Competitive
Biological and medical sciences
Cell Wall - immunology
Cell wall glycoprotein
Epitopes
Fundamental and applied biological sciences. Psychology
Glycoproteins - immunology
Immunoblot
Immunofluorescence
Mannans - immunology
Microbiology
Miscellaneous
Monoclonal antibody
Mycology
Saccharomyces - immunology
Saccharomyces cerevisiae
Species Specificity
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Beschreibung
Zusammenfassung:Murine monoclonal antibodies (mAbs) were selected against a cell wall glycoprotein of Saccharomyces cerevisiae. One of the mAbs (92‐276/018) specifically identified S. cerevisiae and the sibling species S. paradoxus, S. pastorianus and S. bayanus in immunofluorescence studies and immunoblot analyses, while no other yeast genera except Saccharomyces were recognized. Further analysis indicated that the mAb 92‐276/018 reacts with an epitope in the carbohydrate chain of the cell wall glycoproteins.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1994.tb06844.x