Vasopressin and sex differences in hamster flank marking

Vasopressin (AVP) within the medial preoptic-anterior hypothalamic continuum (MPOA-AH) plays an essential role in the control of flank marking in Syrian hamsters. Sex differences are found in the scent marking of many mammalian species, including hamsters. The first two experiments tested the hypothesis that sex differences in flank marking are the result of sex differences in the availability of AVP for release in several CNS sites. No support for this hypothesis was provided because neither immunohistochemical analysis nor radioimmunoassay of tissue punches revealed sex differences in AVP immunoreactivity in the MPOA-AH or other sites likely to be involved in flank marking. The third experiment, which tested the hypothesis that sex differences in flank marking are the result of sex differences in the sensitivity or response of the MPOA-AH to AVP, found no sex differences in the amount of flank marking stimulated by microinjection of AVP in the MPOA-AH. These data provide no support for the hypothesis that sex differences in vasopressinergic activity are responsible for sex differences in flank marking.