Some properties of DNA-dependent RNA polymerase from Streptomyces granaticolor

Some properties of DNA-dependent RNA polymerase from Streptomyces granaticolor

RNA nucleotidyltransferase from Streptomyces granaticolor was purified and some of its properties were investigated. The temperature optimum of the enzyme is 35 degrees C, pH optimum 7.8-8.4. Antibodies against the beta subunit of the enzyme from Bacillus subtilis immunologically cross-react with th...

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Veröffentlicht in:Folia microbiologica 1985-01, Vol.30 (6), p.517-520
Hauptverfasser: RYSAVY, P, SPIZEK, J, KLEGR, M, JANECEK, J, NAPRSTEK, J, HOUSTEK, J
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus subtilis - enzymology
Bacteriology
Biological and medical sciences
Cross Reactions
DNA-Directed RNA Polymerases - metabolism
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Immune Sera
Kinetics
Metabolism. Enzymes
Microbiology
Streptomyces - enzymology
Thermodynamics
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Zusammenfassung:RNA nucleotidyltransferase from Streptomyces granaticolor was purified and some of its properties were investigated. The temperature optimum of the enzyme is 35 degrees C, pH optimum 7.8-8.4. Antibodies against the beta subunit of the enzyme from Bacillus subtilis immunologically cross-react with the beta subunit of the enzyme from S. granaticolor. Antibodies against the beta' subunit of the enzyme from B. subtilis immunologically cross-react with both beta and beta' subunits of the enzyme from S. granaticolor.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02927616