Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein J$_\kappa
The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its c...
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Veröffentlicht in: | Science (American Association for the Advancement of Science) 1994-07, Vol.265 (5168), p.92-95 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJ$_\kappa$. Contrary to previous reports, CBF1-RBPJ$_\kappa$ did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter. |
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ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.8016657 |