Mediation of Epstein-Barr Virus EBNA2 Transactivation by Recombination Signal-Binding Protein J$_\kappa

The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its c...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1994-07, Vol.265 (5168), p.92-95
Hauptverfasser: Henkel, Thomas, Ling, Paul D., Hayward, S. Diane, Peterson, Michael Gregory
Format: Artikel
Sprache:eng
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Zusammenfassung:The Epstein-Barr virus (EBV) transactivator protein, termed Epstein-Barr virus nuclear antigen 2 (EBNA2), plays a critical role in the regulation of latent viral transcription and in the immortalization of EBV-infected B cells. Unlike most transcription factors, EBNA2 does not bind directly to its cis-responsive DNA element but requires a cellular factor, termed C-promoter binding factor 1 (CBF1). Here, CBF1 was purified and was found to directly interact with EBNA2. CBF1 is identical to a protein thought to be involved in immunoglobulin gene rearrangement, RBPJ$_\kappa$. Contrary to previous reports, CBF1-RBPJ$_\kappa$ did not bind to the recombination signal sequences but instead bound to sites in the EBV C-promoter and in the CD23 promoter.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8016657