Identification of drug glucuronides in human urine by RP-HPLC after derivatization

A method for the identification of four types of drug glucuronides in human urine is presented. The approach involves solid-phase extraction (C 18, columns) from acidified human urine and subsequent methylation and acetylation of the extracted drug glucuronides to triacetyl methyl derivatives. These...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1994-02, Vol.12 (2), p.249-254
Hauptverfasser:	Kohl, Christopher, Oelschlager, Herbert, Rothley, Dietrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biological and medical sciences Chromatography, High Pressure Liquid - methods clofibrate Derivatives of drug glucuronides disulfiram General pharmacology Glucuronates - urine Humans Male Medical sciences Pharmacology. Drug treatments phenazone RP-HPLC sulfamethoxazole
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Zusammenfassung:	A method for the identification of four types of drug glucuronides in human urine is presented. The approach involves solid-phase extraction (C 18, columns) from acidified human urine and subsequent methylation and acetylation of the extracted drug glucuronides to triacetyl methyl derivatives. These derivatives were identified by RP-HPLC by comparison with synthesized authentic reference compounds. The scope of the method was demonstrated by identification of glucuronides formed by metabolism of clofibrate. phenazone, disulfiram and sulfamethoxazole in urine samples of two male volunteers.
ISSN:	0731-7085 1873-264X
DOI:	10.1016/0731-7085(94)90036-1