Cloning and sequencing of the chicken anaemia virus (CAV) ORF-3 gene, and the development of an ELISA for the detection of serum antibody to CAV

Chicken anaemia virus (CAV) is a small, unclassified virus involved in anaemia and suspected of causing immunosuppression in young chickens. We have developed an ELISA for the detection of serum antibody to CAV based on cloned antigen. The gene for ORF-3 (the putative capsid protein) was cloned, seq...

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Veröffentlicht in:	Veterinary microbiology 1994-03, Vol.39 (1), p.167-178
Hauptverfasser:	Pallister, Jackie, Fahey, Kevin J., Sheppard, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence ANAEMIA ANEMIA ANEMIE ANIMAL VIRUSES Animals ANTIBODIES Antibodies, Viral - blood ANTICORPS ANTICUERPOS Base Sequence Biological and medical sciences Capsid - chemistry Capsid - genetics Capsid - immunology chicken anemia virus Chicken anemia virus - genetics Chicken anemia virus - immunology CHICKENS Cloning, Molecular DIAGNOSIS DIAGNOSTIC DIAGNOSTICO DNA, Viral - chemistry ELISA Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Genes, Viral Genetics Microbiology Molecular Sequence Data Neutralization Tests Open Reading Frames - genetics Open Reading Frames - immunology POLLO Polymerase Chain Reaction POULET Specific Pathogen-Free Organisms TEST ELISA Virology VIRUS DE LOS ANIMALES VIRUS DES ANIMAUX
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Zusammenfassung:	Chicken anaemia virus (CAV) is a small, unclassified virus involved in anaemia and suspected of causing immunosuppression in young chickens. We have developed an ELISA for the detection of serum antibody to CAV based on cloned antigen. The gene for ORF-3 (the putative capsid protein) was cloned, sequenced and expressed in a bacterial expression system, pGEX. An ORF-3 fusion protein was used to produce an indirect ELISA.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/0378-1135(94)90097-3