An improved method for rapid fixation and embedding of pteridophyte plant materials

A rapid method for fixation and embedding of plant materials, especially pterido-phytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with...

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Veröffentlicht in:	Biotechnic & histochemistry 1994-03, Vol.69 (2), p.99-101
Hauptverfasser:	Devi, S, Lall, V.K, Garg, P, Saxena, A, Tiwari, M
Format:	Artikel
Sprache:	eng
Schlagworte:	AGENT PATHOGENE ANGIOSPERMAS ANGIOSPERME ANGIOSPERMS bacteria Biological and medical sciences BIOLOGICAL TECHNIQUES Cacodylic Acid Cell physiology Fixatives Fundamental and applied biological sciences. Psychology fungi General aspects, methods Glutaral HISTOLOGY INFECTIONS ORGANISMOS PATOGENOS Organometallic Compounds Osmium Tetroxide PATHOGENESE PATHOGENESIS PATHOGENS PATOGENESIS Plant Cells Plant Diseases Plant physiology and development PLANT TISSUES PROCESAMIENTO PROCESSING PTERIDOPHYTA pteridophytes. tannic acid rapid fixation and embedding STRAINING TANINOS TANNIN TANNINS TEJIDOS VEGETALES TISSU VEGETAL Tissue Embedding Tissue Fixation TRAITEMENT
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Beschreibung
Zusammenfassung:	A rapid method for fixation and embedding of plant materials, especially pterido-phytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with fungi and bacteria.
ISSN:	1052-0295 1473-7760
DOI:	10.3109/10520299409106268