Expression of env gene of bovine leukemia virus in rodent cells

The BamHI-BamHI fragment of the env gene of bovine leukemia virus (BLV) cloned in pMMEx expression vector was transfected into Chinese hamster cells. Monoclonal antibodies (MAbs) directed against both conformational and sequential epitopes of gp51 of BLV recognized viral polypeptides expressed in ha...

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Veröffentlicht in:	Archives of virology 1994-01, Vol.135 (1/2), p.201-207
Hauptverfasser:	Zajac, V, Slavikova, K, Babusikova,O
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animals Biological and medical sciences Blotting, Southern Blotting, Western Bovine leukemia virus Cell Line coat proteins Cricetinae Cricetulus cultured cells DNA, Viral - isolation & purification DNA, Viral - metabolism Enzyme-Linked Immunosorbent Assay epitopes Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Gene Products, env - analysis Gene Products, env - biosynthesis Genes, env Genetics genomics hamsters Leukemia Virus, Bovine - genetics Leukemia Virus, Bovine - metabolism Lung Microbiology monoclonal antibodies neutralization tests Restriction Mapping Transfection viral antigens Virology virus neutralization
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Beschreibung
Zusammenfassung:	The BamHI-BamHI fragment of the env gene of bovine leukemia virus (BLV) cloned in pMMEx expression vector was transfected into Chinese hamster cells. Monoclonal antibodies (MAbs) directed against both conformational and sequential epitopes of gp51 of BLV recognized viral polypeptides expressed in hamster cells in Western blotting and enzyme-linked immunosorbent assay.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01309779