Isolation and characterization of an extrachromosomal element from Nocardia mediterranei

Isolation and characterization of an extrachromosomal element from Nocardia mediterranei

Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a lowcopy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685....

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Veröffentlicht in:Plasmid 1985-09, Vol.14 (2), p.126-133
Hauptverfasser: Moretti, Paola, Hintermann, Gilberto, Hütter, Ralf
Format: Artikel
Sprache:eng
Schlagworte:
AISLAMIENTO (TECNICA)
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Applied microbiology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chromosome Mapping
CLONACION
CLONAGE
CLONING
Cloning, Molecular
Conjugation, Genetic
DNA Restriction Enzymes
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
General aspects
Genetic Vectors
Genetics
ISOLATION
ISOLEMENT
Microbiology
Mission oriented research
NOCARDIA
Nocardia - genetics
Nucleic Acid Hybridization
PLASMIDE
PLASMIDIOS
PLASMIDS
Streptomyces - genetics
VECTEUR
VECTORES
VECTORS
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Beschreibung
Zusammenfassung:Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a lowcopy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization experiments showed that pMEA 100 is present in strain LBG A3136 in the free as well as in the integrated form whereas in strain ATCC 13685 only an integrated form was detected. Excision and reintegration in strain LBG A3136 seemed to be site specific. pMEA100 was found to be self-transmissible, eliciting the lethal zygosis phenotype, and is possibly involved in fertility in N. mediterranei.
ISSN:0147-619X
1095-9890
DOI:10.1016/0147-619X(85)90072-1