Determination of octadecadienoic acids in human serum : a critical appraisal

The method based on high-performance liquid chromatography currently used to determine the lipid levels of octadeca-9(Z),11(E)-dienoic acid in human serum fails to distinguish between this acid and octadeca-10(E),12(Z)-dienoic acid. Adducts with the fluorescent dienophile 4-[3-(1-propenyl)propyl]-1,...

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Veröffentlicht in:	Analyst (London) 1994, Vol.119 (3), p.393-396
Hauptverfasser:	READ, G, RICHARDSON, N. R, WICKENS, D. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of complex biological substances Analytical, structural and metabolic biochemistry Biological and medical sciences Blood and biological fluids Chromatography, Gas Chromatography, High Pressure Liquid Fatty Acids, Unsaturated - blood Fundamental and applied biological sciences. Psychology Humans Spectrophotometry, Ultraviolet
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Zusammenfassung:	The method based on high-performance liquid chromatography currently used to determine the lipid levels of octadeca-9(Z),11(E)-dienoic acid in human serum fails to distinguish between this acid and octadeca-10(E),12(Z)-dienoic acid. Adducts with the fluorescent dienophile 4-[3-(1-propenyl)propyl]-1,2,4-triazoline-3,5-dione permit the separation of these two isomeric acids as does gas-liquid chromatography of their methyl esters, but these alternatives produce equivocal results when applied to biological samples.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/AN9941900393