Insulin and interleukin-1 differentially regulate pp63, an acute phase phosphoprotein in hepatoma cell line

We have reported previously that the phosphoprotein pp63, an acute phase protein, which has been recently identified as the rat fetuin, was capable of blocking the mitogenic effect of insulin on the rat Fao hepatoma cell line, without affecting metabolic effects of the hormone. Only the phosphorylat...

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Veröffentlicht in:The Journal of biological chemistry 1994-06, Vol.269 (22), p.15925-15930
Hauptverfasser: Akhoundi, C, Amiot, M, Auberger, P, Le Cam, A, Rossi, B
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container_end_page 15930
container_issue 22
container_start_page 15925
container_title The Journal of biological chemistry
container_volume 269
creator Akhoundi, C
Amiot, M
Auberger, P
Le Cam, A
Rossi, B
description We have reported previously that the phosphoprotein pp63, an acute phase protein, which has been recently identified as the rat fetuin, was capable of blocking the mitogenic effect of insulin on the rat Fao hepatoma cell line, without affecting metabolic effects of the hormone. Only the phosphorylated form of the protein has been shown to exhibit both anti-tyrosine kinase and growth inhibitory properties. In this study, we used the FTO-2B rat hepatoma cell line to analyze the mechanisms involved in the control of synthesis and/or phosphorylation of pp63. For this purpose, we investigated the action of effectors known to modulate hepatic functions, such as cytokines (interleukin (IL)-1 beta and IL-6), which regulate the production of acute phase proteins, and insulin, which elicits profound effects on hepatocyte metabolism. Here, we demonstrate that IL-1 beta diminished markedly the pp63 production by affecting its mRNA transcription and that the cytokine was able to modify the N-glycosylation process of the protein. In contrast, insulin did not affect the biosynthesis of pp63 but dramatically decreased its extent of phosphorylation.
doi_str_mv 10.1016/S0021-9258(17)40769-1
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Acute-Phase Proteins - biosynthesis
Acute-Phase Proteins - isolation & purification
alpha-Fetoproteins - biosynthesis
alpha-Fetoproteins - isolation & purification
Animals
Cell Line
Cell Nucleus - metabolism
Cells, Cultured
Dexamethasone - pharmacology
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Neoplastic
Glycosylation
Humans
Insulin - pharmacology
Interleukin-1 - pharmacology
Interleukin-6 - pharmacology
Kinetics
Liver - drug effects
Liver - metabolism
Liver Neoplasms, Experimental
Male
Phosphoproteins - biosynthesis
Phosphoproteins - isolation & purification
Phosphorylation
Protein Processing, Post-Translational
Rats
Rats, Wistar
Recombinant Proteins - pharmacology
RNA, Messenger - biosynthesis
RNA, Messenger - metabolism
Transcription, Genetic
Tumor Cells, Cultured
title Insulin and interleukin-1 differentially regulate pp63, an acute phase phosphoprotein in hepatoma cell line
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