A latex agglutination test for the qualitative detection of Clostridium perfringens epsilon toxin

A latex agglutination test (LAT), with a neutralisation control, has been developed for the detection of Clostridium perfringens epsilon toxin. The lat readily detects the toxin qualitatively in the intestinal contents of animals suspected of dying from enterotoxaemia. When the lat was compared with...

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Veröffentlicht in:	Research in veterinary science 1994-03, Vol.56 (2), p.259-261
Hauptverfasser:	Martin, P.K., Naylor, R.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - isolation & purification Bacteriology Biological and medical sciences Clostridium perfringens - isolation & purification Enterotoxemia - diagnosis Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology Latex Fixation Tests - methods Latex Fixation Tests - veterinary Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	A latex agglutination test (LAT), with a neutralisation control, has been developed for the detection of Clostridium perfringens epsilon toxin. The lat readily detects the toxin qualitatively in the intestinal contents of animals suspected of dying from enterotoxaemia. When the lat was compared with an enzyme-linked immunosorbent assay ( elisa), the lat proved easy to perform and had a sensitivity and specificity only slightly less than the elisa.
ISSN:	0034-5288 1532-2661
DOI:	10.1016/0034-5288(94)90114-7