Escherichia coli K48 capsular polysaccharide: a glycan containing a novel diacetamido sugar

The structure of the exocellular capsular polysaccharide expressed by Escherichia coli O8:K48:H9 has been investigated by hydrolysis and methylation analysis, and by NMR spectroscopic studies of the polysaccharide and of the oligosaccharides generated by solvolysis with anhydrous HF. The capsular po...

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Veröffentlicht in:	Carbohydrate research 1994-04, Vol.256 (2), p.289-301
Hauptverfasser:	Whittaker, Darryl V., Parolis, Lesley A.S., Parolis, Haralambos
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Bacterial Antigens, Surface - chemistry Bacterial Capsules Bacteriology Biological and medical sciences Carbohydrate Sequence Escherichia coli - chemistry Fundamental and applied biological sciences. Psychology Magnetic Resonance Spectroscopy Microbiology Molecular Sequence Data Morphology, structure, chemical composition Polysaccharides, Bacterial - chemistry Sequence Analysis Stereoisomerism
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Zusammenfassung:	The structure of the exocellular capsular polysaccharide expressed by Escherichia coli O8:K48:H9 has been investigated by hydrolysis and methylation analysis, and by NMR spectroscopic studies of the polysaccharide and of the oligosaccharides generated by solvolysis with anhydrous HF. The capsular polysaccharide was shown to have the repeating unit: ▪ where β- l-Sug p represents 2,3-diacetamido-2,3,6-trideoxy-β- l-mannopyranose.
ISSN:	0008-6215 1873-426X
DOI:	10.1016/0008-6215(94)84214-0