An improved HPLC method to purify erythrocyte cholesterol for estimation of in vivo cholesterol synthesis using the deuterium method

An improved HPLC method to purify erythrocyte cholesterol for estimation of in vivo cholesterol synthesis using the deuterium method

We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from 2H 2O. The new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to m...

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Veröffentlicht in:Applied radiation and isotopes 1994-04, Vol.45 (4), p.529-533
Hauptverfasser: Wong, William W., Hachey, David L., Clarke, Lucinda L., Zhang, Shide, Llaurador, Marisol, Pond, Wilson G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cholesterol - biosynthesis
Cholesterol - blood
Chromatography, High Pressure Liquid - methods
Deuterium
Erythrocytes - metabolism
Female
Swine
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Zusammenfassung:We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from 2H 2O. The new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to maintain the analytical purity of the isolated cholesterol.
ISSN:0969-8043
1872-9800
DOI:10.1016/0969-8043(94)90121-X