A possible osmodependent protease in Escherichia coli

When a strain of Escherichia coli, expressing a hybrid protein GalK‐β‐Gal, is shifted to high osmolarity, the β‐galactosidase activity strongly decreases within 20 min of shock. The loss of β‐galactosidase activity results from degradation of the hybrid protein under osmotic stress. The results rais...

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Veröffentlicht in:	FEMS microbiology letters 1994-03, Vol.116 (3), p.287-291
1. Verfasser:	Meury, Jean
Format:	Artikel
Sprache:	eng
Schlagworte:	Action of physical and chemical agents on bacteria Bacteriology beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences Endopeptidases - biosynthesis Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Galactokinase - genetics Galactokinase - metabolism Gene Expression Genes, Bacterial Microbiology Osmolarity Osmosis - physiology Osmotic Pressure Plasmids - genetics Proteolysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism
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Beschreibung
Zusammenfassung:	When a strain of Escherichia coli, expressing a hybrid protein GalK‐β‐Gal, is shifted to high osmolarity, the β‐galactosidase activity strongly decreases within 20 min of shock. The loss of β‐galactosidase activity results from degradation of the hybrid protein under osmotic stress. The results raise the possibility that osmotic stress induces a specific osmodependent protease.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1994.tb06717.x