Expression of the vasopressin and oxytocin genes in human hypothalami

Poly(A) + RNA isolated from post-mortem human hypothalami has been used to characterize the polyprotein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immunoprecipitation with antibodies raised against either vasopressin or neurophysin identified a product of M r 19000 (prepro-vasopressin). A second less intense product of M r 16500 was tentatively identified as prepro-oxytocin. A cDNA library derived from the human hypothalamic poly(A) + RNA was screened for vasopressin and oxytocin-encoding cDNA using heterologous probes; clones encoding the two precursors were identified and found to be organized as their rat and bovine counterparts. Northern blot analysis shows that the mRNAs for the two prepro-hormones consist of ~ 840 (AVP) and ~ 700 (OT) nucleotides.