Expression of the gag gene of human T-cell leukemia virus type I in Escherichia coli and its diagnostic use

An expression plasmid, pHY202, was constructed which directs the synthesis of a fusion protein encoded by the gag sequence of human T-cell leukemia virus type I (HTLV-I) inserted into the lacZ′ gene. Escherichia coli cells harboring pHY202 produced the 43-kDal LacZ′ -Gag fusion protein with a yield of approx. 0.3 % of total soluble proteins. The fusion protein is specifically recognized by monoclonal antibodies against the Gag proteins p19 and p24, and could be applicable for the diagnosis of HTLV-I infection, because almost all sera from HTLV-I carriers gave a positive response in the enzyme-linked immunosorbent assay (ELISA) employing the LacZ'-Gag hybrid protein purified by immunoaffinity column chromatography.