Production of Diphtheria Toxin by the Submerged Culture Method

THE success which has attended the submerged culture method for the production of penicillin has led us to inquire into the possibilities of producing diphtheria toxin by similar methods. The first step was to see if diphtheria toxin could be produced in flasks which were continually shaken so as to obtain growth throughout the medium instead of merely on the surface.