Modified methods for preparation of cryostat sections of skeletal muscle

Modified methods for preparation of cryostat sections of skeletal muscle

We developed two modified methods for the preparation of cryostat sections of skeletal muscle: one using solid carbon dioxide and the other using the ultra‐low freezer to prepare 2‐methylbutane (isopentane) for freezing of skeletal muscle. The results were quite satisfactory, with good preservation...

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Veröffentlicht in:Muscle & nerve 1985-10, Vol.8 (8), p.664-666
Hauptverfasser: Wu, Ju-Sung, Hogan, Gwendolyn R., Morris, Jerry D.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphatases - analysis
Adult
Animals
Biological and medical sciences
Freezing
Fundamental and applied biological sciences. Psychology
Histological Techniques
Humans
Muscles - cytology
Muscles - enzymology
Muscles - pathology
Rats
Rats, Inbred Strains
Staining and Labeling
Striated muscle. Tendons
Tissue Preservation
Vertebrates: osteoarticular system, musculoskeletal system
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Beschreibung
Zusammenfassung:We developed two modified methods for the preparation of cryostat sections of skeletal muscle: one using solid carbon dioxide and the other using the ultra‐low freezer to prepare 2‐methylbutane (isopentane) for freezing of skeletal muscle. The results were quite satisfactory, with good preservation of cellular details and histochemical properties. Freezing artifacts were minimal when compared with the standard liquid nitrogen method.
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.880080807