Detection of alkaline phosphatase in venom by blotting methods

Detection of alkaline phosphatase in venom by blotting methods. Toxicon 32, 227–231, 1994.—Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The enzyme gave strong reactions in some venoms, but was absent in other venoms, some within the same species. The mol...

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Veröffentlicht in:	Toxicon (Oxford) 1994-02, Vol.32 (2), p.227-231
Hauptverfasser:	Acosta, Alice, Rael, Eppie D., Maddux, Nanci L., Lieb, Carl S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agkistrodon Alkaline Phosphatase - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Crotalid Venoms - enzymology Crotalus Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Isoelectric Focusing
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Zusammenfassung:	Detection of alkaline phosphatase in venom by blotting methods. Toxicon 32, 227–231, 1994.—Venom alkaline phosphatase was detected using a blotting method following electrophoresis. The enzyme gave strong reactions in some venoms, but was absent in other venoms, some within the same species. The mol. wt of the enzyme is close to 100,000 and its p I is between 3.6 and 4.8. The enzyme was inactivated by EDTA and 2-mercaptoethanol, and lost activity by freezing and thawing. Endogenous venom alkaline phosphatase can interfere with alkaline phosphatase-based detection methods. Pre-screening for endogenous venom alkaline phosphatase is recommended prior to using alkaline phosphatase-based detection methods when studying snake venom.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/0041-0101(94)90112-0