Membrane protein from rabbit T-lymphocytes, specifically binding staphylococcal enterotoxin a (SEA)

1. 1. The presence of specific binding of SEA with membranes of lymphocytes from rabbit thymus is established. 2. 2. Components of a glycolipid nature are absent in the composition of the receptor complex for SEA on T-lymphocytes. 3. 3. Suitable conditions for the solubilization of the receptor memb...

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Veröffentlicht in:	International journal of biochemistry 1985, Vol.17 (7), p.781-786
Hauptverfasser:	Ezepchuk, Yu.V., Noskov, A.N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Binding, Competitive Cell Membrane - metabolism Enterotoxins - metabolism Kinetics Membrane Proteins - metabolism Rabbits Receptors, Antigen, T-Cell - metabolism T-Lymphocytes - immunology Thymus Gland - metabolism
Online-Zugang:	Volltext
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Zusammenfassung:	1. 1. The presence of specific binding of SEA with membranes of lymphocytes from rabbit thymus is established. 2. 2. Components of a glycolipid nature are absent in the composition of the receptor complex for SEA on T-lymphocytes. 3. 3. Suitable conditions for the solubilization of the receptor membrane fraction by Triton X-100 are described. 4. 4. The SEA-binding membrane fraction is isolated by means of an affinity-chromatography method. 5. 5. The main component of the fraction is a protein with molecular mass 42 kd. 6. 6. The isolated protein inhibits the specific binding of [ 125I] SEA on cell (T-lymphocytes) and subcell (membrane) levels.
ISSN:	0020-711X
DOI:	10.1016/0020-711X(85)90264-2